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Untapped indigenous PSB potential from forest ecosystem for enhancing soil enzyme activity and agronomic traits of different wheat genotypes

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Abstract

Phosphate solubilizing bacteria (PSB) are well known potential candidate for improving soil health and thus plant growth promotion. Impact of PSB isolated from forest ecosystem on wheat crop still remains a major area to explore. Present study involves the screening of 18 PSB recovered from three Dalbergia sissoo provenances (Lachhiwala, Tanakpur and Pantnagar) of Uttarakhand, India against three wheat genotypes (PBW 502, HD 2329 and UP 262) under pot trial condition. PSB inoculation positively influences wheat agronomic traits and promote three soil enzyme activities e.g. alkaline phosphatase, fluorescein di acetate hydrolysis and urease which considered as soil health indicators. Maximum phosphate solubilizing index (3.6 cm) and zinc solubilizing index (2.8 cm) was observed in strain P2. Maximum soluble P concentration was observed in strain P2 (81.1 mg mL−1) followed by P4 (56 mg mL−1) and L3 (50.46 mg mL−1). Significant pH drop were observed during P solubilization due to production of organic acid which is a principal mechanism to solubilize inorganic P which further validated via Fourier transform infrared spectroscopy. R-software analysis revealed that response of PSB inoculation were genotype-specific whereas principal component analysis results in selection of six efficient PSB strains e.g., Pseudomonas paralactis-L3, Klebsiella variicola-L4, Pseudomonas sp.-P2, Kitasatospora sp.-T1, Streptomyces sp.-T3 and Micrococcus sp.-T4.

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Data availability

All data underlying results are available as part of article and no additinal source data is required.

Abbreviations

PSB:

Phosphate solubilizing bacteria

PSI:

Phosphate solubilizing index

ZSI:

Zinc solubilizing index

PGP:

Plant growth-promoting

AP:

Alkaline phosphatase

FDA:

Fluorescein di acetate hydrolysis

PCA:

Principal component analysis

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Acknowledgements

Authors thanks to A.F.R.C (Agroforestry Research Center) G.B.P UAT, Pantnagar, Uttarakhand, India for providing poly house facilities.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Microbiology, G.B. Pant University of Agriculture and Technology, Pantnagar, India

    Hemant Dasila, Vandana Jaggi & Manvika Sahgal

  2. Department of Microbiology, Akal College of Basic Sciences, Eternal University, Sirmaur, Himachal Pradesh, India

    Hemant Dasila

  3. Department of Agronomy, G.B. Pant University of Agriculture and Technology, Pantnagar, India

    V. K. Sah

  4. Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, India

    Gohar Taj

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  1. Hemant Dasila
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Contributions

HD: original draft preparation, data analysis, methodology and software. MS: conceptualization, Supervision and editing, VKS: visualization and editing, GT: validation and reviewing, VJ: data validation and literature search.

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Correspondence to Manvika Sahgal.

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Dasila, H., Sah, V.K., Jaggi, V. et al. Untapped indigenous PSB potential from forest ecosystem for enhancing soil enzyme activity and agronomic traits of different wheat genotypes. Vegetos (2023). https://doi.org/10.1007/s42535-023-00638-w

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